High generation of reactive oxygen species from neutrophils in patients with severe COVID-19.
Veenith, Tonny Martin, Helena Le Breuilly, Martin Whitehouse, Tony Gao-Smith, Fang Duggal, Niharika Lord, Janet M Mian, Rubina Sarphie, David Moss, Paul
Veenith, Tonny
Martin, Helena
Le Breuilly, Martin
Whitehouse, Tony
Gao-Smith, Fang
Duggal, Niharika
Lord, Janet M
Mian, Rubina
Sarphie, David
Moss, Paul
Citations
Altmetric:
Affiliation
Other Contributors
Publication date
2022-06-21
Subject
Collections
Research Projects
Organizational Units
Journal Issue
Abstract
eutrophilia and an elevated neutrophil:lymphocyte ratio are both characteristic features of severe COVID-19 infection. However, functional neutrophil responses have been poorly investigated in this setting. We utilised a novel PMA-based stimulation assay to determine neutrophil-derived reactive oxygen species (ROS) generation in patients with severe COVID-19 infection, non-COVID related sepsis and healthy study participants. ROS production was markedly elevated in COVID-19 patients with median values ninefold higher than in healthy controls and was particularly high in patients on mechanical ventilation. ROS generation correlated strongly with neutrophil count and elevated levels were also seen in patients with non-COVID related sepsis. Relative values, adjusted for neutrophil count, were high in both groups but extreme low or high values were seen in two patients who died shortly after testing, potentially indicating a predictive value for neutrophil function. Our results show that the high levels of neutrophils observed in patients with COVID-19 and sepsis exhibit functional capacity for ROS generation. This may contribute to the clinical features of acute disease and represents a potential novel target for therapeutic intervention.
Citation
Veenith T, Martin H, Le Breuilly M, Whitehouse T, Gao-Smith F, Duggal N, Lord JM, Mian R, Sarphie D, Moss P. High generation of reactive oxygen species from neutrophils in patients with severe COVID-19. Sci Rep. 2022 Jun 21;12(1):10484. doi: 10.1038/s41598-022-13825-7
Type
Article
Other
Other