Photobiomodulation reduces hippocampal apoptotic cell death and produces a Raman spectroscopic "signature".
Davies, David J Hadis, Mohammed Di Pietro, Valentina Lazzarino, Giuseppe Forcione, Mario Harris, Georgia Stevens, Andrew R Soon, Wai Cheong Goldberg Oppenheimer, Pola Milward, Michael
Davies, David J
Hadis, Mohammed
Di Pietro, Valentina
Lazzarino, Giuseppe
Forcione, Mario
Harris, Georgia
Stevens, Andrew R
Soon, Wai Cheong
Goldberg Oppenheimer, Pola
Milward, Michael
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Affiliation
University of Birmingham; University of Catania
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Publication date
2022-03-03
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Abstract
Apoptotic cell death within the brain represents a significant contributing factor to impaired post-traumatic tissue function and poor clinical outcome after traumatic brain injury. After irradiation with light in the wavelength range of 600-1200 nm (photobiomodulation), previous investigations have reported a reduction in apoptosis in various tissues. This study investigates the effect of 660 nm photobiomodulation on organotypic slice cultured hippocampal tissue of rats, examining the effect on apoptotic cell loss. Tissue optical Raman spectroscopic changes were evaluated. A significantly higher proportion of apoptotic cells 62.8±12.2% vs 48.6±13.7% (P<0.0001) per region were observed in the control group compared with the photobiomodulation group. After photobiomodulation, Raman spectroscopic observations demonstrated 1440/1660 cm-1 spectral shift. Photobiomodulation has the potential for therapeutic utility, reducing cell loss to apoptosis in injured neurological tissue, as demonstrated in this in vitro model. A clear Raman spectroscopic signal was observed after apparent optimal irradiation, potentially integrable into therapeutic light delivery apparatus for real-time dose metering.
Citation
Davies DJ, Hadis M, Di Pietro V, Lazzarino G, Forcione M, Harris G, Stevens AR, Soon WC, Goldberg Oppenheimer P, Milward M, Belli A, Palin WM. Photobiomodulation reduces hippocampal apoptotic cell death and produces a Raman spectroscopic "signature". PLoS One. 2022 Mar 3;17(3):e0264533. doi: 10.1371/journal.pone.0264533.
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Article