Structural biology of SARS-CoV-2.
Zaidi, Asiya Kamber Dawoodi, Sunny
Zaidi, Asiya Kamber
Dawoodi, Sunny
Citations
Altmetric:
Affiliation
Other Contributors
Publication date
2024-01-03
Subject
Collections
Research Projects
Organizational Units
Journal Issue
Abstract
This chapter provides a comprehensive overview of the techniques employed to unravel the structural biology of SARS-CoV-2, facilitating a deeper understanding of the virus for developing future therapeutic strategies. Various techniques such as Electron microscopy (EM) for capturing high-resolution images of the virus and X-ray crystallography used for determining atomic-level structures of viral proteins are discussed. Cryo-electron microscopy (cryo-EM) imaging is also examined as a powerful tool for visualizing the virus's structure in its native state. Intracellular detection and tracking of SARS-CoV-2 are discussed, highlighting the techniques employed to study the virus's behavior within host cells. The chapter further explores how cryo-EM has been instrumental in delivering high-quality structural information on SARS-CoV-2, enabling researchers to better understand its mechanisms of infection and replication. The structural visualization of SARS-CoV-2 is then presented, focusing on key components such as the spike protein structure, RNA polymerase structure, and the visualization of intact and in-situ virions using cryo-electron tomography (cryo-ET). Lastly, the chapter touches upon the application of nuclear magnetic resonance (NMR) spectroscopy for studying the dynamics and interactions of viral proteins.
Citation
Zaidi AK, Dawoodi S. Structural biology of SARS-CoV-2. Prog Mol Biol Transl Sci. 2024;202:31-43. doi: 10.1016/bs.pmbts.2023.11.001.
Type
Article