The microbial composition of pancreatic ductal adenocarcinoma: A systematic review of 16S rRNA gene sequencing.
Merali, Nabeel Chouari, Tarak Sweeney, Casie Halle-Smith, James Maria-Danae, Jessel Wang, Bing O' Brien, James Patel, Bhavik Suyama, Satoshi Jiménez, José I
Merali, Nabeel
Chouari, Tarak
Sweeney, Casie
Halle-Smith, James
Maria-Danae, Jessel
Wang, Bing
O' Brien, James
Patel, Bhavik
Suyama, Satoshi
Jiménez, José I
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2024-06-14
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Abstract
Background: Pancreatic cancer, specifically pancreatic ductal adenocarcinoma (PDAC), continues to pose a significant clinical and scientific challenge. The most significant finding of recent years is that PDAC tumours harbour their specific microbiome, which differs amongst tumour entities and is distinct from healthy tissue. This review aims to evaluate and summarise all PDAC studies that have used the next-generation technique, 16S rRNA gene amplicon sequencing within each bodily compartment. As well as establishing a causal relationship between PDAC and the microbiome.
Materials and methods: This systematic review was carried out according to the Preferred Reporting Items for Systematic Reviews and Meta-analysis (PRISMA) guidelines. A comprehensive search strategy was designed, and 1727 studies were analysed.
Results: In total, 38 studies were selected for qualitative analysis and summarised significant PDAC bacterial signatures. Despite the growing amount of data provided, we are not able to state a universal 16S rRNA gene microbial signature that can be used for PDAC screening. This is most certainly due to the heterogeneity of the presentation of results, lack of available datasets and the intrinsic selection bias between studies.
Conclusion: Several key studies have begun to shed light on causality and the influence the microbiome constituents and their produced metabolites could play in tumorigenesis and influencing outcomes. The challenge in this field is to shape the available microbial data into targetable signatures. Making sequenced data readily available is critical, coupled with the coordinated standardisation of data and the need for consensus guidelines in studies investigating the microbiome in PDAC.
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Merali N, Chouari T, Sweeney C, Halle-Smith J, Maria-Danae J, Wang B, O' Brien J, Patel B, Suyama S, Jiménez JI, Roberts KJ, Velliou E, Sivakumar S, Rockall TA, Demirkan A, Pedicord V, Deng D, Giovannetti E, Annels NE, Frampton AE. The microbial composition of pancreatic ductal adenocarcinoma: A systematic review of 16S rRNA gene sequencing. Int J Surg. 2024 Jun 14. doi: 10.1097/JS9.0000000000001762.
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