Cerebrospinal fluid polymerase chain reaction in the diagnosis of neonatal bacterial meningitis: a single-center experience from Vietnam
Nga Nguyen, Thi Quynh Nguyen, Thi Van Tran, Minh Dien Skinner, Alyson Narchi, Hassib
Nga Nguyen, Thi Quynh
Nguyen, Thi Van
Tran, Minh Dien
Skinner, Alyson
Narchi, Hassib
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Affiliation
Hanoi Medical University, and Neonatal Centre; Vietnam National Children's Hospital; Walsall Healthcare NHS Trust; College of Medicine and Health Sciences, United Arab Emirates University, Al Ain, United Arab Emirates;
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Publication date
2022-10-29
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Abstract
Objective: To compare the performance of cerebrospinal fluid (CSF) polymerase chain reaction (PCR) with bacterial culture for the diagnosis of neonatal bacterial meningitis (NBM).
Method: The CSF analysis of neonate with confirmed bacterial meningitis was performed with PCR and bacterial culture, and results were compared.
Result: Among 24 neonates, the pathogens Identified included E. coli K1, GBS, Streptococcus pneumoniae and Listeria. PCR identified 20 (83.3%) pathogens, and culture 4 (16.7%) pathogens. Prior antibiotics were administered to 20 (83.3%) neonates in whom PCR identified 17 (85%) and culture 3 (15%) pathogens.
Conclusion: CSF PCR had a higher yield of pathogens than CSF culture in confirmed neonatal bacterial meningitis with a high rate of prior antibiotic therapy.
Citation
Nga Nguyen TQ, Nguyen TV, Tran MD, Skinner A, Narchi H. Cerebrospinal Fluid Polymerase Chain Reaction in the Diagnosis of Neonatal Bacterial Meningitis: A Single-Center Experience From Vietnam. Indian Pediatr. 2022 Dec 15;59(12):943-945. Epub 2022 Oct 29. PMID: 36333894.
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Article