Inter- and intra-observer agreement of PD-L1 SP142 scoring in breast carcinoma-a large multi-institutional international study.
Author
Zaakouk, MohamedVan Bockstal, Mieke
Galant, Christine
Callagy, Grace
Provenzano, Elena
Hunt, Roger
D'Arrigo, Corrado
Badr, Nahla M
O'Sullivan, Brendan
Starczynski, Jane
Tanchel, Bruce
Mir, Yasmeen
Lewis, Paul
Shaaban, Abeer M
Publication date
2023-02-28
Metadata
Show full item recordAbstract
The assessment of PD-L1 expression in TNBC is a prerequisite for selecting patients for immunotherapy. The accurate assessment of PD-L1 is pivotal, but the data suggest poor reproducibility. A total of 100 core biopsies were stained using the VENTANA Roche SP142 assay, scanned and scored by 12 pathologists. Absolute agreement, consensus scoring, Cohen's Kappa and intraclass correlation coefficient (ICC) were assessed. A second scoring round after a washout period to assess intra-observer agreement was carried out. Absolute agreement occurred in 52% and 60% of cases in the first and second round, respectively. Overall agreement was substantial (Kappa 0.654-0.655) and higher for expert pathologists, particularly on scoring TNBC (6.00 vs. 0.568 in the second round). The intra-observer agreement was substantial to almost perfect (Kappa: 0.667-0.956), regardless of PD-L1 scoring experience. The expert scorers were more concordant in evaluating staining percentage compared with the non-experienced scorers (R2 = 0.920 vs. 0.890). Discordance predominantly occurred in low-expressing cases around the 1% value. Some technical reasons contributed to the discordance. The study shows reassuringly strong inter- and intra-observer concordance among pathologists in PD-L1 scoring. A proportion of low-expressors remain challenging to assess, and these would benefit from addressing the technical issues, testing a different sample and/or referring for expert opinions.Citation
Zaakouk M, Van Bockstal M, Galant C, Callagy G, Provenzano E, Hunt R, D'Arrigo C, Badr NM, O'Sullivan B, Starczynski J, Tanchel B, Mir Y, Lewis P, Shaaban AM. Inter- and Intra-Observer Agreement of PD-L1 SP142 Scoring in Breast Carcinoma-A Large Multi-Institutional International Study. Cancers (Basel). 2023 Feb 28;15(5):1511. doi: 10.3390/cancers15051511Type
ArticlePMID
36900303Journal
CancersPublisher
MDPIae974a485f413a2113503eed53cd6c53
10.3390/cancers15051511