Novel detection of specific bacterial quorum sensing molecules in saliva: Potential non-invasive biomarkers for pulmonary Pseudomonas aeruginosa in cystic fibrosis
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Author
Webb, KarmelCámara, Miguel
Zain, Nur Masirah M
Halliday, Nigel
Bruce, Kenneth D
Nash, Edward F
Whitehouse, Joanna L
Knox, Alan
Forrester, Douglas
Smyth, Alan R
Williams, Paul
Fogarty, Andrew
Barr, Helen L
Publication date
2021-09-11Subject
Public health. Health statistics. Occupational health. Health educationPharmacology
Clinical pathology
Biochemistry
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Pseudomonas aeruginosa produces specific signalling molecules, 2-alkyl-4-quinolones (AQs) that are detectable in the sputum of adults with cystic fibrosis (CF) and who have pulmonary infection with this opportunistic pathogen. This study aimed to determine whether AQs could be detected in saliva of patients with CF and known infection with Pseudomonas aeruginosa. Saliva and sputum samples were obtained from 89 adults with CF and analyzed using liquid chromatography-tandem mass spectrometry. AQs were detected in 39/89 (43.8%) saliva samples and 70/77(90.9%) sputum samples. Salivary AQs had a sensitivity of 50% (95%CI; 37.8; 62.2), specificity of 100% (95%CI; 47.8; 100), when compared to a molecular microbiological measure of P. aeruginosa in sputum as measured using polymerase chain reaction. Specific AQs produced by P. aeruginosa can be detected in the saliva and warrant investigation as potential non-invasive biomarkers of pulmonary P. aeruginosa.Citation
Webb K, Cámara M, Zain NMM, Halliday N, Bruce KD, Nash EF, Whitehouse JL, Knox A, Forrester D, Smyth AR, Williams P, Fogarty A, Barr HL. Novel detection of specific bacterial quorum sensing molecules in saliva: Potential non-invasive biomarkers for pulmonary Pseudomonas aeruginosa in cystic fibrosis. J Cyst Fibros. 2022 Jul;21(4):626-629. doi: 10.1016/j.jcf.2021.08.030. Epub 2021 Sep 11Type
ArticleAdditional Links
http://www.sciencedirect.com/science/journal/15691993PMID
34518117Journal
Journal of Cystic FibrosisPublisher
Elsevierae974a485f413a2113503eed53cd6c53
10.1016/j.jcf.2021.08.030