Fecal microbiota and volatile metabolome pattern alterations precede late-onset meningitis in preterm neonates.
Author
Frerichs, Nina MDeianova, Nancy
El Manouni El Hassani, Sofia
Acharjee, Animesh
Quraishi, Mohammed Nabil
de Boode, Willem P
Cossey, Veerle
Hulzebos, Christian V
van Kaam, Anton H
Kramer, Boris W
d'Haens, Esther
de Jonge, Wouter J
Vijlbrief, Daniel C
van Weissenbruch, Mirjam M
Daulton, Emma
Wicaksono, Alfian N
Covington, James A
Benninga, Marc A
de Boer, Nanne K H
van Goudoever, Johannes B
Niemarkt, Hendrik J
de Meij, Tim G J
Publication date
2024-05-23Subject
Communicable diseases
Metadata
Show full item recordAbstract
Objective: The fecal microbiota and metabolome are hypothesized to be altered before late-onset neonatal meningitis (LOM), in analogy to late-onset sepsis (LOS). The present study aimed to identify fecal microbiota composition and volatile metabolomics preceding LOM. Methods: Cases and gestational age-matched controls were selected from a prospective, longitudinal preterm cohort study (born <30 weeks' gestation) at nine neonatal intensive care units. The microbial composition (16S rRNA sequencing) and volatile metabolome (gas chromatography-ion mobility spectrometry (GC-IMS) and GC-time-of-flight-mass spectrometry (GC-TOF-MS)), were analyzed in fecal samples 1-10 days pre-LOM. Results: Of 1397 included infants, 21 were diagnosed with LOM (1.5%), and 19 with concomitant LOS (90%). Random Forest classification and MaAsLin2 analysis found similar microbiota features contribute to the discrimination of fecal pre-LOM samples versus controls. A Random Forest model based on six microbiota features accurately predicts LOM 1-3 days before diagnosis with an area under the curve (AUC) of 0.88 (n=147). Pattern recognition analysis by GC-IMS revealed an AUC of 0.70-0.76 (P<0.05) in the three days pre-LOM (n=92). No single discriminative metabolites were identified by GC-TOF-MS (n=66). Conclusion: Infants with LOM could be accurately discriminated from controls based on preclinical microbiota composition, while alterations in the volatile metabolome were moderately associated with preclinical LOM.Citation
Frerichs NM, Deianova N, El Manouni El Hassani S, Acharjee A, Quraishi MN, de Boode WP, Cossey V, Hulzebos CV, van Kaam AH, Kramer BW, d'Haens E, de Jonge WJ, Vijlbrief DC, van Weissenbruch MM, Daulton E, Wicaksono AN, Covington JA, Benninga MA, de Boer NKH, van Goudoever JB, Niemarkt HJ, de Meij TGJ. Fecal microbiota and volatile metabolome pattern alterations precede late-onset meningitis in preterm neonates. J Infect Dis. 2024 May 23:jiae265. doi: 10.1093/infdis/jiae265. Epub ahead of print.Type
ArticleAdditional Links
https://academic.oup.com/jidPMID
38781449Publisher
Oxford University Pressae974a485f413a2113503eed53cd6c53
10.1093/infdis/jiae265