The potential of fecal microbiota and amino acids to detect and monitor patients with adenoma.
Author
Bosch, SofieAcharjee, Animesh
Quraishi, Mohammed N
Rojas, Patricia
Bakkali, Abdellatif
Jansen, Erwin Ew
Brizzio Brentar, Marina
Kuijvenhoven, Johan
Stokkers, Pieter
Struys, Eduard
Beggs, Andrew D
Gkoutos, Georgios V
de Meij, Tim Gj
de Boer, Nanne Kh
Publication date
2022-01
Metadata
Show full item recordAbstract
The risk of recurrent dysplastic colonic lesions is increased following polypectomy. Yield of endoscopic surveillance after adenoma removal is low, while interval colorectal cancers occur. To longitudinally assess the dynamics of fecal microbiota and amino acids in the presence of adenomatous lesions and after their endoscopic removal. In this longitudinal case-control study, patients collected fecal samples prior to bowel preparation before scheduled colonoscopy and 3 months after this intervention. Based on colonoscopy outcomes, patients with advanced adenomas and nonadvanced adenomas (0.5-1.0 cm) who underwent polypectomy during endoscopy (n = 19) were strictly matched on age, body-mass index, and smoking habits to controls without endoscopic abnormalities (n = 19). Microbial taxa were measured by 16S RNA sequencing, and amino acids (AA) were measured by high-performance liquid chromatography (HPLC). Adenoma patients were discriminated from controls based on AA and microbial composition. Levels of proline (p = .001), ornithine (p = .02) and serine (p = .02) were increased in adenoma patients compared to controls but decreased to resemble those of controls after adenoma removal. These AAs were combined as a potential adenoma-specific panel (AUC 0.79(0.64-0.94)). For bacterial taxa, differences between patients with adenomas and controls were found (Bifidobacterium spp.↓, Anaerostipes spp.↓, Butyricimonas spp.↑, Faecalitalea spp.↑ and Catenibacterium spp.↑), but no alterations in relative abundance were observed after polypectomy. Furthermore, Faecalitalea spp. and Butyricimonas spp. were significantly correlated with adenoma-specific amino acids. We selected an amino acid panel specifically increased in the presence of adenomas and a microbial signature present in adenoma patients, irrespective of polypectomy. Upon validation, these panels may improve the effectiveness of the surveillance program by detection of high-risk individuals and determination of surveillance endoscopy timing, leading to less unnecessary endoscopies and less interval cancer.Citation
Bosch S, Acharjee A, Quraishi MN, Rojas P, Bakkali A, Jansen EE, Brizzio Brentar M, Kuijvenhoven J, Stokkers P, Struys E, Beggs AD, Gkoutos GV, de Meij TG, de Boer NK. The potential of fecal microbiota and amino acids to detect and monitor patients with adenoma. Gut Microbes. 2022 Jan-Dec;14(1):2038863. doi: 10.1080/19490976.2022.2038863Type
ArticleOther
Additional Links
http://www.tandfonline.com/kgmiPMID
35188868Journal
Gut MicrobesPublisher
Taylor & Francisae974a485f413a2113503eed53cd6c53
10.1080/19490976.2022.2038863