Fecal Microbiota Transplantation (FMT) in Clostridium difficile Infection: A Paradigm Shift in Gastrointestinal Microbiome Modulation
Hamza Saeed, Muhammad Qamar, Sundas Ishtiaq, Ayesha Umaira Khan, Qudsia Atta, Asma Atta, Maryam Ishtiaq, Hifza Khan, Marriam Saeed, Muhammad R Iqbal, Ayesha
Hamza Saeed, Muhammad
Qamar, Sundas
Ishtiaq, Ayesha
Umaira Khan, Qudsia
Atta, Asma
Atta, Maryam
Ishtiaq, Hifza
Khan, Marriam
Saeed, Muhammad R
Iqbal, Ayesha
Affiliation
The Dudley Group NHS Foundation Trust; Foundation University Medical College Islamabad; Combined Military Hospital Lahore Medical College and Institute of Dentistry et al
Other Contributors
Publication date
2025/05/29
Subject
Collections
Research Projects
Organizational Units
Journal Issue
Abstract
Clostridium difficile (C. difficile) infection (CDI) poses a tremendous clinical challenge, especially in patients with recurrent disease and antibiotic resistance. Fecal microbiota transplantation (FMT) has become a new therapeutic strategy for restoring gut microbiota and decreasing CDI recurrence. The study aims to assess the clinical effectiveness of FMT in adult subjects with recurrent or refractory CDI, determine its effect on gut microbiome diversity, and track safety outcomes and rates of recurrence post-treatment. FMT was compared against standard antibiotic treatments to establish its efficacy in decreasing infection persistence and improving patients' quality of life. This study examines the efficacy, safety, and modulation of microbiota by FMT in an ensemble of 250 patients diagnosed with CDI, with equal gender distribution and a mean age of 55.61. Among the study participants, 131 (52.4%) underwent FMT by various routes of administration, including 66 (25.2%) through colonoscopy, 73 (29.2%) via a nasogastric tube, 60 (24.0%) via enema, and 54 (21.6%) through oral capsule administration. The success rate for FMT was reported as 88 (35.2%), partial success at 74 (29.6%), and treatment failure at 88 (35.2%). CDI recurrence was reported in 130 (52.0%) of patients after FMT. The gut microbiome enhanced diversity, measured in terms of the Shannon Diversity Index, increased significantly from 3.96 before FMT to 5.88 after FMT, thus indicating a favorable impact on gut microbial composition. Furthermore, 132 (52.8%) converted from C. difficile polymerase chain reaction (PCR) toxin positive to negative, corroborating successful pathogen clearance. On secondary outcomes, the quality of life in patients improved in 90 (36%), antibiotic dependence was reduced in 88 (35.2%), and hospitalization was lessened in 72 (28.8%). Inflammatory markers, such as white blood cell (WBC) counts and C-reactive protein (CRP), went downward but did not reach statistical significance. Logistic regression analysis identified age, severity of CDI, and prior exposure to antibiotics as the main predictors for the efficacy of FMT (p < 0.05). It is concluded that FMT is a promising alternative treatment for recurrent CDI through modulation of gut microbiota and decreasing the severity of infection. Future work is, however, required to establish treatment protocols with optimized results for long-term effectiveness and minimized recurrence risks.
Citation
Hamza Saeed M, Qamar S, Ishtiaq A, Umaira Khan Q, Atta A, Atta M, Ishtiaq H, Khan M, Saeed MR, Iqbal A. Fecal Microbiota Transplantation (FMT) in Clostridium difficile Infection: A Paradigm Shift in Gastrointestinal Microbiome Modulation. Cureus. 2025 May 29;17(5):e85054. doi: 10.7759/cureus.85054